Prof. Dr. Kai Johnsson

Coordinating functions and editorial work

2014 - Scientific Advisory Board, Quartet Medicines, Boston, USA
2012 - Editorial Advisory Board of Science
2012 - Member of the school-wide tenure committee of EPFL
2010 - Editorial Board of Chemical Society Reviews
2010 - Co-Director of the Swiss-wide research program NCCR Chemical Biology (budget of SFR 4 million per year)
2009 - Member of the steering committee of Institute of Chemical Sciences and Engineering, EPFL
2008 - Editorial Board of ChemBioChem
2006 - Editorial Board of Chemistry&Biology
2005 - Associate Editor of ACS Chemical Biology
2003 - 2010 - Board of Directors, Covalys Biosciences, Switzerland

Most important publications

1. Yu, Q.; Xue, L.; Hiblot, J.; Griss, R.; Fabritz, S.; Roux, C.; Binz, P. A.; Haas, D.; Okun, J. G.; Johnsson, K. (2018) Semisynthetic sensor proteins enable metabolic assays at the point of care. Science, 361, 1122-1126

2. Sallin, O.; Reymond, L.; Gondrand, C.; Raith, F.; Koch, B.; Johnsson, K. (2018) Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife, 7

3. Lukinavicius, G.; Reymond, L.; D'Este, E.; Masharina, A.; Gottfert, F.; Ta, H.; Guther, A.; Fournier, M.; Rizzo, S.; Waldmann, H.; Blaukopf, C.; Sommer, C.; Gerlich, D. W.; Arndt, H. D.; Hell, S. W.; Johnsson, K. (2014) Fluorogenic probes for live-cell imaging of the cytoskeleton. Nature Methods, 11, 731

4. Haruki, H.; Pedersen, M. G.; Gorska, K. I.; Pojer, F.; Johnsson, K. (2013) Tetrahydrobiopterin biosynthesis as an off-target of sulfa drugs. Science, 340, 987

5. Lukinavicius, G.; Umezawa, K.; Olivier, N.; Honigmann, A.; Yang, G.; Plass, T.; Mueller, V.; Reymond, L.; Correa, I. R., Jr.; Luo, Z. G.; Schultz, C.; Lemke, E. A.; Heppenstall, P.; Eggeling, C.; Manley, S.; Johnsson, K. (2013) A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chemistry, 5, 132

6. Chidley, C.; Haruki, H.; Pedersen, M. G.; Muller, E.; Johnsson, K. (2017) A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis. Nature Chem Biol, 7, 375

7. Gautier, A.; Juillerat, A.; Heinis, C.; Correa, I. R.; Kindermann, M.; Beaufils, F.; Johnsson, K. (2008) An engineered protein tag for multiprotein labeling in living cells. Chem&Biol, 15, 128

8. George, N.; Pick, H.; Vogel, H.; Johnsson, N.; Johnsson, K. (2004) Specific labeling of cell surface proteins with chemically diverse compounds” J Am Chem Soc, 126, 8896

9. Keppler, A.; Pick, H.; Arrivoli, C.; Vogel, H.; Johnsson, K. (2004) Labeling of fusion proteins with synthetic fluorophores in live cells. P Natl Acad Sci USA, 101, 9955

10. Keppler, A.; Gendreizig, S.; Gronemeyer, T.; Pick, H.; Vogel, H.; Johnsson, K. (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol, 21, 86

Patents

1. “Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample” Kai Johnsson, Alberto Schena, Rudolf Griss (2013) PCT/EP2013/065223;

2. "Cell permeable, fluorescent dye" Kai Johnsson, Grazvydas Lukinavicius, Keitaro Umezawa, (2011) PCT/EP2011/064750; licensed and exploited by Spirochrome.

3. “Labelling of fusion proteins with synthetic probes” Gautier, Arnaud; Johnsson, Kai; Kindermann, Maik; Juillerat, Alexandre; Beaufils, Florent, PCT Int. Appl. (2008) WO 2008/012296;

4. “Methods for cell-free protein display” Florian Hollfelder, Viktor Stein, Andrew Griffiths, Kai Johnsson. UK0714698.9 (2007)

5. “Mutants of human O6-alkylguanine-DNA alkyltransferase with improved properties”; Barnikov, Jan; Chidley, Christopher; Gronemeyer, Thomas; Heinis, Christian; Jaccard, Hughes; Johnsson, Kai; Juillerat, Alexandre; Keppler, Antje. PCT Int. Appl. (2005), WO 2005085431;

6. “Method for identification of fragmentation sites in a reporter protein in the development of split protein sensors” Johnsson, Kai; Tafelmeyer, Petra. PCT Int. Appl. (2005), WO 2005038050;

7. “Labeling of fusion proteins by enzymic incorporation of a coenzyme A derivative into an acyl carrier protein moiety”; Johnsson, Kai; George, Nathalie. PCT Int. Appl. (2004), WO 2004104588;

8. “Guanine derivatives as substrates for O6-alkylguanine-DNA alkyltransferase and their use in labeling the enzyme and fusion proteins”; Kindermann, Maik; Johnsson, Kai; Bieri, Christoph. PCT Int. Appl. (2004), WO 2004031405;

9. “O6-alkylguanine-DNA alkyltransferases for efficient labeling of fusion proteins”; Juillerat, Alexandre; Keppler, Antje; Johnsson, Kai; Gronemeier, Thomas; Gendreizig, Susanne; Brecht, Andreas. PCT Int. Appl. (2004), WO 2004031404;

10. “Methods using O6-alkylguanine-DNA alkyltransferases”; Kiddle, Simon John; Johnsson, Kai; Gendreizig, Susanne; Keppler, Antje. PCT Int. Appl. (2002), WO 2002083937;