Publications

Single event visualization of unconventional secretion of FGF2

Dimou, E., Cosentino, K., Platonova, E., Ros, U., Sadeghi, M., Kashyap, P., Katsinelos, T., Wegehingel, S., Noé, F., García-Sáez ,A. J., Ewers, H., Nickel, W. (2018)

J Cell Biol. 2019 Feb 4;218(2):683-699. Epub 2018 Nov 23.

DOI: 10.1083/jcb.201802008

Quantitative analysis of circadian single cell oscillations in response to temperature

Abraham, U., Schlichting, J. K., Kramer, A., Herzel, H.

PLoS One. 2018 Jan 2;13(1):e0190004.

DOI: 10.1371/journal.pone.0190004

Ultradian Rhythms in the Transcriptome of Neurospora crassa

Ananthasubramaniam, B., Diernfellner, A., Brunner, M., Herzel, H.

iScience. 2018 Nov 30;9:475-486. Epub 2018 Nov 10.

DOI: 10.1016/j.isci.2018.11.012

Regulation of synaptic release-site-Ca2+-channel coupling as a mechanism to control release probability and short-term plasticity

Bohme, M. A., Grasskamp, A. T., and Walter, A. M.

FEBS Lett. 2018 Nov;592(21):3516-3531. Epub 2018 Jul 31.

DOI: 10.1002/1873-3468.13188

Synaptotagmin-1 drives synchronous Ca2+-triggered fusion by C2B-domain-mediated synaptic-vesicle-membrane attachment.

Chang, S., Trimbuch, T., Rosenmund, C.

Nat Neurosci. 2018 Jan;21(1):33-40. Epub 2017 Dec 11.

DOI: 10.1038/s41593-017-0037-5

Unconventional mechanisms of eukaryotic protein secretion

Dimou, E., Nickel, W.

Curr Biol. 2018 Apr 23;28(8):R406-R410. 

DOI: 10.1016/j.cub.2017.11.074

Open-source recombinant monoclonal secondary nanobodies

Ewers, H.

J Cell Biol. 2018 Mar 5;217(3):809-811. Epub 2018 Feb 14.

DOI: 10.1083/jcb.201802025

Optical tools for understanding the complexity of beta cell signaling and insulin release

Frank, J., Broichhagen, J., Yushchenko, D., Trauner, D., Schultz, C., Hodson, D.

Nat Rev Endocrinol. 2018 Dec;14(12):721-737.

DOI: 10.1038/s41574-018-0105-2

Surface Immobilization of Viruses and Nanoparticles Elucidates Early Events in Clathrin-mediated Endocytosis

Fratini, M., Wiegand, T., Funaya, C., Spatz, J. P., Cavalcanti-Adam, E. A., Boulant, S.

ACS Infect Dis. 2018 Nov 9;4(11):1585-1600. Epub 2018 Sep 26.

DOI: 10.1021/acsinfecdis.8b00134

Expansion Stimulated Emission Depletion Microscopy (ExSTED)

Gao, M., Maraspini, R., Beutel, O., Zehtabian, A., Eickholt, B., Honigmann, A., Ewers, H.

ACS Nano. 2018 May 22;12(5):4178-4185. Epub 2018 Apr 19.

DOI: 10.1021/acsnano.8b00776

Endogenous Fatty Acids are Essential Autocrine Signaling Factors of Pancreatic β-Cells and Insulin Secretion

Hauke, S., Keutler, K., Phapale, P., Yushchenko, D., Schultz, C.

Diabetes. 2018 Oct;67(10):1986-1998.

doi.org/10.2337/db17-1215.

The Evolution of Variability and Robustness in Neural Development

Hiesinger, P. R., Hassan, B. A.

Trends in Neuroscience 41(9):577-586

DOI: 10.1016/j.tins.2018.05.007

The where, what, and when of membrane protein degradation in neurons

Jin, E. J., Kiral, F. R., Hiesinger, P. R.

Dev Neurobiol. 2018 Mar;78(3):283-297. doi: 10.1002/dneu.22534. Epub 2017 Sep 19. Review.

Live observation of two parallel membrane degradation pathways at axon terminals

Jin, E. J., Kiral, F. R., Özel, M. N., Burchardt, L. S., Osterland, M., Epstein, D., Wolfenberg, H., Prohaska, S., Hiesinger, P. R.

Curr. Biol., 28(7):1027-1038.e4

DOI: 10.1016/j.cub.2018.02.032

PTEN suppresses axon outgrowth by down-regulating the level of detyrosinated microtubules

Kath, C., Goni-Oliver, P., Müller, R., Schultz, C., Haucke, V., Eickholt, B., Schmoranzer, J.

PLoS One 13, e0193257 (2018). 

DOI: 10.1371/journal.pone.0193257

Unconventional secretion mediates the trans-cellular spreading of tau

Katsilenos, T., Zeitler, M., Dimou, E., Karakatsani, A., Müller, H. M., Nachman, E., Steringer, J. P., Ruiz de Almodovar, C., Nickel, W., Jahn, T. R.

Cell Rep 23:2039-2055.

DOI: 10.1016/j.celrep.2018.04.056

Rab GTPases and Membrane Trafficking in Neurodegeneration

Kiral, F. K., Kohrs, F., Jin, E. J. and Hiesinger, P. R.

Curr Biol. 2018 Apr 23;28(8):R471-R486. 

DOI: 10.1016/j.cub.2018.02.010

A potent and selective PARP11 inhibitor suggests coupling between cellular localization and catalytic activity

Kirby, I. T., Kojic, A., Arnold, M. R., Thorsell, A.-G., Karlberg, T., Vermehren-Schmaedick, A., Sreenivasan, R., Schultz, C., Schüler, H., Cohen M. S.

Cell Chem Biol. 2018 Dec 20;25(12):1547-1553.e12. Epub 2018 Oct 18.

DOI: 10.1016/j.chembiol.2018.09.011

Novel lipid tools and probes for biological investigations

Laguerre, A., Schultz, C.

Curr Opin Cell Biol. 2018 Aug;53:97-104. Epub 2018 Jul 14. Review.

DOI: 10.1016/j.ceb.2018.06.013

VAMPnets for deep learning of molecular kinetics

Mardt, A, Pasquali, L., Wu, H., Noé, F.

Nature Communicationsvolume 9, Article number: 5 (2018) (https://www.nature.com/articles/s41467-017-02388-1)

DOI: 10.1038/s41467-017-02388-1

Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasomal inhibition

Nowak, K., Taubert, R. M., Haberecht, S., Venz, S., Krüger, E.

Biosci Rep. 2018 Sep 3. pii: BSR20180393. [Epub ahead of print]

DOI: 10.1042/BSR20180393

Control of SUMO and Ubiquitin by ROS: Signaling and disease implications.

Stankovic-Valentin, N., Melchior, F.

Mol Aspects Med. 63, 3-17. (Review)

DOI: 10.1016/j.mam.2018.07.002

Autoregulation of class II alpha PI3K activity by its lipid binding PX-C2 domain module

Wang, H., Lo, W. T., Vujičić Žagar, A., Gulluni, F., Scapozza, L., Haucke, V.*, Vadas, O.*

Mol. Cell, 71, 343-351(*co-corresponding authors)

DOI: 10.1016/j.molcel.2018.06.042

Phosphoinositide conversion in endocytosis and in the endolysosomal system

Wallroth, A., Haucke, V.

J Biol Chem. 2018 Feb 2;293(5):1526-1535. Epub 2017 Dec 27.

DOI: 10.1074/jbc.R117.000629

Deep Generative Markov State Models

Wu, H., Mardt, A., Pasquali, L., Noé, F.

32nd Conference on Neural Information Processing Systems (NeurIPS 2018), Montréal, Canada

An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1beta

Brough, D., Pellegrin, P., and Nickel, W.

J Cell Sci 130:3197-3202

DOI: 10.1242/jcs.204206

The coding and noncoding transcriptome of Neurospora crassa

Cemel, I. A., Ha, N., Schermann, G., Yonekawa, S., Brunner, M.

BMC Genomics, 18(1), 978

DOI: 10.1186/s12864-017-4360-8

Optical control of GPR40 signalling in pancreatic ß-cells

Frank, J. A., Yushchenko, D. A., Fine, N. H. F., Duca, M., Citir, M., Broichhagen, J., Hodson, D. J., Schultz, C., Trauner, D.

Chem. Sci. 8, 7604-7610 (2017)

DOI: 10.1039/c7sc01475a

Trifunctional lipid probes for comprehensive studies of single lipid species in living cells

Höglinger, D., Nadler, A., Haberkant, P., Kirkpatrick, J., Schifferer, M., Stein, F., Hauke, S., Porter, F. D., Schultz, C.

Proc. Natl. Acad. Sci. USA 114, 1566-1571

DOI: 10.1073/pnas.1611096114

Reciprocal regulation of carbon monoxide metabolism and the circadian clock.

Klemz, R., Reischl, S., Wallach, T., Witte, N., Jürchott, K., Klemz, S., Lang, V., Lorenzen, S., Knauer, M., Heidenreich, S., Xu, M., Ripperger, J. A., Schupp, M., Stanewsky, R., Kramer, A.

Nat Struct Mol Biol. 2017 Jan;24(1):15-22

DOI: 10.1073/pnas.1611096114

Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases.

Lauinger, L., Li, J., Shostak, A., Cemel, I. A., Ha, N., Zhang, Y., Merkl, P. E., Obermeyer, S., Stankovic-Valentin, N., Schafmeier, T., Wever, W. J., Bowers, A. A., Carter, K. P., Palmer, A. E., Tschochner, H., Melchior, F., Deshaies, R. J., Brunner, M., Diernfellner, A.

Nat Chem Biol. 2017 Jul;13(7):709-714

DOI: 10.1038/nchembio.2370

mTORC1 activity repression by late endosomal phosphatidylinositol 3,4-bisphosphate.

Marat, A. L., Wallroth, A., Lo, W. T., Müller, R., Norata, G. D., Falasca, M., Schultz, C., Haucke, V.

Science. 2017 Jun 2;356(6341):968-972

DOI: 10.1126/science.aaf8310

 

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.

Piwecka, M., Glažar, P., Hernandez-Miranda, L. R., Memczak, S., Wolf, S. A., Rybak-Wolf, A., Filipchyk, A., Klironomos, F., Cerda Jara, C. A., Fenske, P., Trimbuch, T., Zywitza, V., Plass, M., Schreyer, L., Ayoub, S., Kocks, C., Kühn, R., Rosenmund, C., Birchmeier, C., Rajewsky, N.

Science. 2017 Sep 22;357(6357). pii: eaam8526

DOI: 10.1126/science.aam8526

Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission.

Reddy-Alla, S., Böhme, M. A., Reynolds, E., Beis, C., Grasskamp, A. T., Mampell, M. M., Maglione, M., Jusyte, M., Rey, U., Babikir, H., McCarthy, A. W., Quentin, C., Matkovic, T., Bergeron, D .D., Mushtaq, Z., Göttfert, F., Owald, D., Mielke, T., Hell, S. W., Sigrist, S. J.Walter, A. M.

Neuron. 2017 Sep 13;95(6):1350-1364.e12

DOI: 10.1016/j.neuron.2017.08.016

Flavivirus infection uncouples translation suppression from cellular stress responses

Roth, H., Magg, V., Uch, F., Mutz, P., Klein, P., Haneke, K., Lohmann, V., Bartenschlager, R., Fackler, O.T., Locker, N., Stoecklin, G., Ruggieri, A.

MBio. 2017 Jan 10;8(1). pii: e02150-16

DOI: 10.1128/mBio.02150-16

A Ratiometric Sensor for Imaging Insulin Secretion in Single β Cells

Schifferer, M., Yushchenko, D. A., Hodson, D. J., Bolbat, A., Stein, F., Schultz, C.

Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4

DOI: 10.1016/j.chembiol.2017.03.001

Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.

Steringer, J. P., Lange, S., Čujová, S., Šachl, R., Poojari, C., Lolicato, F., Beutel, O., Müller, H.M., Unger, S., Coskun, Ü., Honigmann, A., Vattulainen I, Hof, M., Freund, C., Nickel, W.

Elife. 2017 Jul 19;6. pii: e28985

DOI: 10.7554/eLife.28985

Principles for circadian orchestration of metabolic pathways.

Thurley, K., Herbst, C., Wesener, F., Koller, B., Wallach, T., Maier B, Kramer, A., Westermark, P. O.

Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1572-1577

DOI: 10.1073/pnas.1613103114

A lipid off-switch for mTORC1

Wallroth, A., Haucke, V.

Mol. Cell. Oncol. 4:e1356899

DOI: 10.1080/23723556.2017.1356899

Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.

Walter, A. M., Müller, R., Tawfik, B., Wierda, K. D., Pinheiro, P. S., Nadler, A., McCarthy, A. W., Ziomkiewicz, I., Kruse, M., Reither, G., Rettig, J., Lehmann, M., Haucke, V., Hille, B., Schultz, C., Sorensen, J. B.

Elife. 2017 Oct 25;6. pii: e30203

DOI: 10.7554/eLife.30203

Photoswitchable diacylglycerols enable optical control of protein kinase C.

Frank, J. A., Yushchenko, D. A., Hodson, D. J., Lipstein, N., Nagpal, J., Rutter, G. A., Rhee, J.-S., Gottschalk, A., Brose, N., Schultz*, C., Trauner*, D.

Nat. Chem. Biol. 12, 755-762 (2016)

DOI: 10.1038/nchembio.2141

Small Molecule Inhibitors Targeting Tec Kinase Block Unconventional Secretion of Fibroblast Growth Factor 2.

La Venuta, G., Wegehingel, S., Sehr, P., Müller, H. M., Dimou, E., Steringer, J. P., Grotwinkel, M., Hentze, N., Mayer, M. P., Will, D. W., Uhrig, U., Lewis, J. D., Nickel, W.

J Biol Chem. 2016 Aug 19;291(34):17787-803

DOI: 10.1074/jbc.M116.729384

Feedback Loops of the Mammalian Circadian Clock Constitute Repressilator.

Pett, J. P., Korenčič, A., Wesener, F., Kramer, A., Herzel, H.

PLoS Comput Biol. 2016 Dec 12;12(12):e1005266

DOI: 10.1371/journal.pcbi.1005266

MHC class II complexes sample intermediate states along the peptide exchange pathway

Wieczorek, M., Sticht, J., Stolzenberg, S., Günther, S., Wehmeyer, C,. El Habre, Z., Alvaro-Benito, M., Noé, F.*, Freund, C*.

Nature Communications 7, 13224 (2016)

DOI: 10.1038/ncomms13224